PUBLICATION
Transition from non-motile behaviour to directed migration during early PGC development in zebrafish
- Authors
- Blaser, H., Eisenbeiss, S., Neumann, M., Reichman-Fried, M., Thisse, B., Thisse, C., and Raz, E.
- ID
- ZDB-PUB-050907-5
- Date
- 2005
- Source
- Journal of Cell Science 118(17): 4027-4038 (Journal)
- Registered Authors
- Blaser, Heiko, Neumann, Marc, Raz, Erez, Reichman-Fried, Michal, Thisse, Bernard, Thisse, Christine
- Keywords
- Chemokine, CXCR4, Danio rerio, Cell migration, EMT, E-cadherin
- MeSH Terms
-
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/physiology
- Cadherins/genetics
- Cadherins/metabolism
- RNA-Binding Proteins
- Animals
- Gene Expression Regulation, Developmental
- Xenopus Proteins/metabolism*
- Caspases/metabolism
- Promoter Regions, Genetic
- Transcription, Genetic
- In Situ Hybridization
- Mutagenesis, Site-Directed
- Chemokine CXCL12
- Animals, Genetically Modified
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Cell Movement/physiology*
- Germ Cells/cytology*
- Germ Cells/physiology*
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Chemokines, CXC/metabolism*
- Cell Shape
- PubMed
- 16129886 Full text @ J. Cell Sci.
Citation
Blaser, H., Eisenbeiss, S., Neumann, M., Reichman-Fried, M., Thisse, B., Thisse, C., and Raz, E. (2005) Transition from non-motile behaviour to directed migration during early PGC development in zebrafish. Journal of Cell Science. 118(17):4027-4038.
Abstract
The migration of zebrafish primordial germ cells (PGCs) is directed by SDF-1a and serves as a model for long-range chemokine-guided cell migration. Whereas the development and migration of zebrafish PGCs have been studied in great detail starting at mid-gastrulation stages when the cells exhibit guided active migration [7-8 hours post fertilization (hpf)], earlier stages have not yet been examined. Here we show that the PGCs acquire competence to respond to the chemokine following discrete maturation steps. Using the promoter of the novel gene askopos and RNA elements of nanos1 to drive GFP expression in PGCs, we found that immediately after their specification (about 3 hpf) PGCs exhibit simple cell shape. This stage is followed by a phase at which the cells assume complex morphology yet they neither change their position nor do they respond to SDF-1a. During the third phase, a transition into a 'migratory stage' occurs as PGCs become responsive to directional cues provided by somatic cells secreting the chemokine SDF-1a. This transition depends on zygotic transcription and on the function of the RNA-binding protein Dead end and is correlated with down regulation of the cell adhesion molecule E-cadherin. These distinctive morphological and molecular alterations could represent a general occurrence in similar processes critical for development and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping