PUBLICATION
Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos
- Authors
- Lee, H.C., Lai, W.L., Lin, C.Y., Zeng, C.W., Sheu, J.C., Chou, T.B., Tsai, H.J.
- ID
- ZDB-PUB-221224-22
- Date
- 2022
- Source
- International Journal of Molecular Sciences 23(24): (Journal)
- Registered Authors
- Lee, Hung-Chieh, Lin, Cheng-Yung, Tsai, Huai-Jen, Zeng, Chin-Wei
- Keywords
- Anp32a, neuronal regeneration, proliferation, spinal cord injury, zebrafish
- MeSH Terms
-
- Animals
- Mammals/metabolism
- Motor Neurons/metabolism
- Nerve Regeneration
- RNA, Messenger/metabolism
- Recovery of Function/physiology
- Spinal Cord/metabolism
- Spinal Cord Injuries*/metabolism
- Transcription Factors/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 36555564 Full text @ Int. J. Mol. Sci.
Citation
Lee, H.C., Lai, W.L., Lin, C.Y., Zeng, C.W., Sheu, J.C., Chou, T.B., Tsai, H.J. (2022) Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos. International Journal of Molecular Sciences. 23(24):.
Abstract
After spinal cord injury (SCI) in mammals, neuronal regeneration is limited; in contrast, such regeneration occurs quickly in zebrafish. Member A of the acidic nuclear phosphoprotein 32 (ANP32a) family is involved in neuronal development, but its function is controversial, and its involvement in zebrafish SCI remains unknown. To determine the role of zebrafish ANP32a in the neuronal regeneration of SCI embryos, we microinjected ANP32a mRNA into embryos from zebrafish transgenic line Tg(mnx1:GFP) prior to SCI. Compared to control SCI embryos, the results showed that the regeneration of spinal cord and resumption of swimming capability were promoted by the overexpression of ANP32a mRNA but reduced by its knockdown. We next combined fluorescence-activated cell sorting with immunochemical staining of anti-GFAP and immunofluorescence staining against anti-PH3 on Tg(gfap:GFP) SCI embryos. The results showed that ANP32a promoted the proliferation and cell number of radial glial cells at the injury epicenter at 24 h post-injury (hpi). Moreover, when we applied BrdU labeling to SCI embryos derived from crossing the Tg(gfap:GFP) and Tg(mnx1:TagRFP) lines, we found that both radial glial cells and motor neurons had proliferated, along with their increased cell numbers in Anp32a-overexpression SCI-embryos. On this basis, we conclude that ANP32a plays a positive role in the regeneration of zebrafish SCI embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping