ZFIN Image: Lee et al., 2022, Figure 6

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Figure 6

The overexpression of Anp32a in SCI embryos of zebrafish could induce an increase in the cell number of motor neuron progenitor cells (pMN) around the SCI site. Fluorescence-activated cell sorting (FACS) and a cell cycle analysis of pMN in Anp32a-overexpressing SCI embryos of zebrafish were performed. The Anp32a mRNA was microinjected into the one-cell stage of embryos from wild-type zebrafish, followed by SCI at 48 hpf. After deheading, suspended cells were stained with antibodies against Olig2 and DAPI at 24 hpi. (A) Scatter profiles of Anp32a-overexpressed SCI-zebrafish embryonic cells. All analytic cells were gated at P1; RFP-labelled Olig2-positive pMN cells were gated at P2; DAPI-labeled-positive cells were gated at P4; Olig2-negative cells were gated at P5. (B) Typical FACS dataset. The number shown in the right corner represents the percentage of cells at each gate relative to the total number of examined cells in each group. (C) Cell cycle analysis from DAPI-labeled-positive cells shown on P4.

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