ZFIN ID: ZDB-PERS-010509-1
Tsai, Huai-Jen
Email: hjtsai@ntu.edu.tw
URL:
Affiliation: Tsai Laboratory
Address: Molecular Biology and Biotecchnology Lab, Mackay Medical College No. 46 Sec. 3, Zhongzheng Rd., Sanzhi Dist. New Taipei City 252, Taiwan
Country:
Phone: +886-2-26360303#1724
Fax: +886-939-919701
ORCID ID:


BIOGRAPHY AND RESEARCH INTERESTS
Prof. Tsai has served as Associate Professor, Institute of Fisheries Science, National Taiwan University, since September of 1988 upon completing his postdoctoral work at Department of Biology, Johns Hopkins University. Since then, he has been working on the Molecular Biology of fish, fish Embryology, Gene Transfer Technology of fish and shellfish and Marine Algae Biotechnology for more than 30 years. His research materials focus on aquatic organisms from different aspects, including basic studies and industrial applications. Up to August of 2019, Prof. Tsai has published a total more than of 130 international refereed papers. He is a recipient of Outstanding Research Award offered by Ministry of Sciences and Technology, Taiwan, for three times.


PUBLICATIONS
Lee, H.C., Chao, H.T., Lee, S.Y., Lin, C.Y., Tsai, H.J. (2023) The Upstream 1350~1250 Nucleotide Sequences of the Human ENDOU-1 Gene Contain Critical Cis-Elements Responsible for Upregulating Its Transcription during ER Stress. International Journal of Molecular Sciences. 24(24):
Zeng, C.W., Sheu, J.C., Tsai, H.J. (2023) Genomic Structure, Protein Character, Phylogenic Implication, and Embryonic Expression Pattern of a Zebrafish New Member of Zinc Finger BED-Type Gene Family. Genes. 14(1):
Lee, H.C., Lai, W.L., Lin, C.Y., Zeng, C.W., Sheu, J.C., Chou, T.B., Tsai, H.J. (2022) Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos. International Journal of Molecular Sciences. 23(24):
Lin, C.Y., Lin, H.Y., Chuang, C.K., Zhang, P.H., Tu, Y.R., Lin, S.P., Tsai, H.J. (2022) Quantification of Idua Enzymatic Activity Combined with Observation of Phenotypic Change in Zebrafish Embryos Provide a Preliminary Assessment of Mutated idua Correlated with Mucopolysaccharidosis Type I. Journal of personalized medicine. 12(8):
Lin, C.Y., Tseng, H.C., Chu, Y.R., Wu, C.L., Zhang, P.H., Tsai, H.J. (2022) Cerebroventricular Injection of Pgk1 Attenuates MPTP-Induced Neuronal Toxicity in Dopaminergic Cells in Zebrafish Brain in a Glycolysis-Independent Manner. International Journal of Molecular Sciences. 23(8):
Lee, H.C., Hsieh, C.C., Tsai, H.J. (2022) KEPI Plays a Negative Role in the Repression that Accompanies Translational Inhibition Guided by the uORF Element of Human chop Transcript during Stress Response. Gene. 817:146160
Zeng, C.W., Sheu, J.C., Tsai, H.J. (2022) Hypoxia-Responsive Subtype Cells Differentiate Into Neurons in the Brain of Zebrafish Embryos Exposed to Hypoxic Stress. Cell transplantation. 31:9636897221077930
Lee, H.C., Lin, C.Y., Tsai, H.J. (2021) Zebrafish, an In Vivo Platform to Screen Drugs and Proteins for Biomedical Use. Pharmaceuticals (Basel, Switzerland). 14(6):
Zeng, C.W., Kamei, Y., Shigenobu, S., Sheu, J.C., Tsai, H.J. (2021) Injury-induced Cavl-expressing cells at lesion rostral side play major roles in spinal cord regeneration. Open Biology. 11:200304
Lee, H.C., Fu, C.Y., Lin, C.Y., Hu, J.R., Huang, T.Y., Lo, K.Y., Tsai, H.Y., Sheu, J.C., Tsai, H.J. (2021) Poly(U)-specific endoribonuclease ENDOU promotes translation of human CHOP mRNA by releasing uORF element-mediated inhibition. The EMBO journal. 40(11):e104123
Lin, C.Y., Lin, H.Y., Chuang, C.K., Zhang, P.H., Tu, R.Y., Lin, S.P., Tsai, H.J. (2020) Effect of Mutated ids Overexpression on IDS Enzyme Activity and Developmental Phenotypes in Zebrafish Embryos: A Valuable Index for Assessing Critical Point-Mutations Associated with Mucopolysaccharidosis Type II Occurrence in Humans. Diagnostics (Basel, Switzerland). 10(10):
Zeng, C.W., Sheu, J.C., Tsai, H.J. (2020) A new member of the forkhead box protein family in zebrafish: Domain composition, phylogenetic implication and embryonic expression pattern. Gene expression patterns : GEP. 35:119093
Zeng, C.W., Sheu, J.C., Tsai, H.J. (2020) The Neuronal Regeneration of Adult Zebrafish After Spinal Cord Injury Is Enhanced by Transplanting Optimized Number of Neural Progenitor Cells. Cell transplantation. 29:963689720903679
Lin, C.Y., Wu, C.L., Lee, K.Z., Chen, Y.J., Zhang, P.H., Chang, C.Y., Han, H.J., Lin, S.Z., Tsai, H.J. (2019) Extracellular Pgk1 enhances neurite outgrowth of motoneurons through Nogo66/NgR-independent targeting of NogoA. eLIFE. 8:
Yang, C.Y., Chen, C., Lin, C.Y., Chen, Y.H., Lin, C.Y., Chi, C.W., Chen, Y.J., Liu, S.C., Chang, T.K., Tang, C.H., Lai, Y.W., Tsai, H.J., Chen, J.J., Wang, S.W. (2019) Garcimultiflorone K inhibits angiogenesis through Akt/eNOS- and mTOR-dependent pathways in human endothelial progenitor cells. Phytomedicine : international journal of phytotherapy and phytopharmacology. 64:152911
Lin, C.Y., Zhang, P.H., Chen, Y.J., Wu, C.L., Tsai, H.J. (2018) Conditional Overexpression of rtn4al in Muscle of Adult Zebrafish Displays Defects Similar to Human Amyotrophic Lateral Sclerosis. Marine biotechnology (New York, N.Y.). 21(1):52-64
Lin, C.Y., Lee, H.C., Wu, J.H., Tsai, H.J. (2018) Short fish-origin DNA elements served as flanking sequences in a knockdown cloning vector enabling the generation of a functional siRNA molecule in mammalian cells and fish embryos. Biochemical and Biophysical Research Communications. 505(3):850-857
Lee, H.C., Fu, C.Y., Zeng, C.W., Tsai, H.J. (2017) Embryonic expression patterns of Eukaryotic EndoU ribonuclease family gene endouC in zebrafish. Gene expression patterns : GEP. 25-26:66-70
Lin, C.Y., He, J.Y., Zeng, C.W., Loo, M.R., Chang, W.Y., Zhang, P.H., Tsai, H.J. (2017) microRNA-206 modulates an Rtn4a/Cxcr4a/Thbs3a axis in newly forming somites to maintain and stabilize the somite boundary formation of zebrafish embryos.. Open Biology. 7(7)
Fu, C.Y., Wang, P.C., Tsai, H.J. (2017) Competitive binding between Seryl-tRNA synthetase/YY1 complex and NFKB1 at the distal segment results in differential regulation of human vegfa promoter activity during angiogenesis. Nucleic acids research. 45:2423-2437
Zeng, C.W., Kamei, Y., Wang, C.T., Tsai, H.J. (2016) Subtypes of hypoxia-responsive cells differentiate into neurons in spinal cord of zebrafish embryos after hypoxic stress. Biology of the cell. 108(12):357-377
Chiang, C.Y., Lin, C.Y., Chen, Y.T., Tsai, H.J. (2016) Blue fluorescent protein derived from the mutated purple chromoprotein isolated from the sea anemone Stichodactyla haddoni. Protein engineering, design & selection : PEDS. 29(11):523–530
Lin, C.Y., Chiang, C.Y., Tsai, H.J. (2016) Zebrafish and Medaka: new model organisms for modern biomedical research. Journal of Biomedical Science. 23:19
Yu, W.H., Ho, Y.L., Huang, P.T., Chu, S.L., Tsai, H.J., Liou, H.H. (2016) The Phosphorylation State of GSK3β Serine 9 Correlated to the Development of Valproic Acid-Associated Fetal Cardiac Teratogenicity, Fetal VPA Syndrome, Rescued by Folic Acid Administration. Cardiovascular Toxicology. 16(1):34-45
Lee, H.C., Su, M.Y., Lo, H.C., Wu, C.C., Hu, J.R., Lo, D.M., Chao, T.Y., Tsai, H.J., Dai, M.S. (2015) Cancer metastasis and EGFR signaling is suppressed by Amiodarone-induced Versican V2. Oncotarget. 6(40):42976-87
Lee, H.C., Lo, H.C., Lo, D.M., Su, M.Y., Hu, J.R., Wu, C.C., Chang, S.N., Dai, M.S., Tsai, C.T., Tsai, H.J. (2015) Amiodarone Induces Overexpression of Similar to Versican b to Repress the EGFR/Gsk3b/Snail Signaling Axis during Cardiac Valve Formation of Zebrafish Embryos. PLoS One. 10:e0144751
Lin, C.Y., Huang, H.Y., Lu, P.N., Lin, C.W., Lu, K.M., Tsai, H.J. (2015) Ras-Related Nuclear Protein is required for late developmental stages of retinal cells in zebrafish eyes. The International journal of developmental biology. 59:435-442
Lee, H.C., Lin, Y.Z., Lai, Y.T., Huang, W.J., Hu, J.R., Tsai, J.N., Tsai, H.J. (2014) Glycogen Synthase Kinase 3 beta in somites plays a role during the angiogenesis of zebrafish embryos. The FEBS journal. 281(19):4367-83
Chiang, C.Y., Chen, Y.L., and Tsai, H.J. (2014) Different Visible Colors and Green Fluorescence Were Obtained from the Mutated Purple Chromoprotein Isolated from Sea Anemone. Marine biotechnology (New York, N.Y.). 16(4):436-46
Candel, S., de Oliveira, S., López-Muñoz, A., García-Moreno, D., Espín-Palazón, R., Tyrkalska, S.D., Cayuela, M.L., Renshaw, S.A., Corbalán-Vélez, R., Vidal-Abarca, I., Tsai, H.J., Meseguer, J., Sepulcre, M.P., Mulero, V. (2014) Tnfa signaling through tnfr2 protects skin against oxidative stress-induced inflammation. PLoS Biology. 12:e1001855
Lee, H.C., Lu, P.N., Huang, H.L., Chu, C., Li, H.P., Tsai, H.J. (2014) Zebrafish transgenic line huORFZ is an effective living bioindicator for detecting environmental toxicants. PLoS One. 9:e90160
Lin, C.Y., Lee, H.C., Chen, H.C., Hsieh, C.C., and Tsai, H.J. (2013) Normal Function of Myf5 During Gastrulation Is Required for Pharyngeal Arch Cartilage Development in Zebrafish Embryos. Zebrafish. 10(4):486-99
Lin, C.Y., Chen, J.S., Loo, M.R., Hsiao, C.C., Chang, W.Y., and Tsai, H.J. (2013) MicroRNA-3906 Regulates Fast Muscle Differentiation through Modulating the Target Gene homer-1b in Zebrafish Embryos. PLoS One. 8(7):e70187
Lin, C.Y., Lee, H.C., Fu, C.Y., Ding, Y.Y., Chen, J.S., Lee, M.H., Huang, W.J., and Tsai, H.J. (2013) miR-1 and miR-206 target different genes to have opposing roles during angiogenesis in zebrafish embryos. Nature communications. 4:2829
Chen, Y.H., Hsu, R.J., Chen, T.Y., Huang, Y.K., Lee, H.C., Hu, S.C., Harn, H.J., Jeng, J.R., Sun, C.K., Lin, S.Z., and Tsai, H.J. (2012) The toxic effect of Amiodarone on valve formation in the developing heart of zebrafish embryos. Reproductive toxicology (Elmsford, N.Y.). 33(2):233-244
Tu, C.T., Yang, T.C., Huang, H.Y., and Tsai, H.J. (2012) Zebrafish arl6ip1 Is Required for Neural Crest Development during Embryogenesis. PLoS One. 7(3):e32899
Huang, H.Y., Liu, J.T., Yan, H.Y., and Tsai, H.J. (2012) Arl6ip1 Plays a Role in Proliferation during Zebrafish Retinogenesis. Cells, tissues, organs. 196(2):161-174
Lee, H.C., Chen, Y.J., Liu, Y.W., Lin, K.Y., Chen, S.W., Lin, C.Y., Lu, Y.C., Hsu, P.C., Lee, S.C., and Tsai, H.J. (2011) Transgenic zebrafish model to study translational control mediated by upstream open reading frame of human chop gene. Nucleic acids research. 39(20):e139
Chang, S.H., Lee, B.C., Chen, Y.D., Lee, Y.C., and Tsai, H.J. (2011) Development of transgenic zooplankton Artemia as a bioreactor to produce exogenous protein. Transgenic Research. 20(5):1099-111
Hami, D., Grimes, A.C., Tsai, H.J., and Kirby, M.L. (2011) Zebrafish cardiac development requires a conserved secondary heart field. Development (Cambridge, England). 138(11):2389-2398
Hsu, R.J., Lin, C.C., Su, Y.F., and Tsai, H.J. (2011) dickkopf-3-related Gene Regulates the Expression of Zebrafish myf5 Gene through Phosphorylated p38a-dependent Smad4 Activity. The Journal of biological chemistry. 286(8):6855-6864
Hsu, R.J., and Tsai, H.J. (2011) Performing the Labeled microRNA Pull-Down (LAMP) Assay System: An Experimental Approach for High-Throughput Identification of microRNA-Target mRNAs. Methods in molecular biology (Clifton, N.J.). 764:241-247
Hsu, R.J., Lin, C.Y., Hoi, H.S., Zheng, S.K., Lin, C.C., and Tsai, H.J. (2010) Novel intronic microRNA represses zebrafish myf5 promoter activity through silencing dickkopf-3 gene. Nucleic acids research. 38(13):4384-4393
Lin, C.Y., Yang, P.H., Kao, C.L., Huang, H.I., and Tsai, H.J. (2010) Transgenic zebrafish eggs containing bactericidal peptide is a novel food supplement enhancing resistance to pathogenic infection of fish. Fish & shellfish immunology. 28(3):419-427
Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W. (2010) Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development. Developmental Biology. 337(2):432-443
Lee, H.C., Tseng, W.A., Lo, F.Y., Liu, T.M., and Tsai, H.J. (2009) FoxD5 mediates anterior-posterior polarity through upstream modulator Fgf signaling during zebrafish somitogenesis. Developmental Biology. 336(2):232-245
Ohn, J., Tsai, H.J., and Liebling, M. (2009) Joint dynamic imaging of morphogenesis and function in the developing heart. Organogenesis. 5(4):166-173
Ho, Y.L., Lin, Y.H., Tsai, W.Y., Hsieh, F.J., and Tsai, H.J. (2009) Conditional Antisense-Knockdown of Zebrafish Cardiac Troponin C as a New Animal Model for Dilated Cardiomyopathy. Circulation journal : official journal of the Japanese Circulation Society. 73(9):1691-1697
Lin, C.Y., Chen, W.T., Lee, H.C., Yang, P.H., Yang, H.J., and Tsai, H.J. (2009) The transcription factor six1a plays an essential role in the craniofacial myogenesis of zebrafish. Developmental Biology. 331(2):152-166
Fu, C.Y., Lee, H.C., and Tsai, H.J. (2009) The Molecular Structures and Expression Patterns of Zebrafish Troponin I Genes. Gene expression patterns : GEP. 9(5):348-356
Hsu, R.J., Yang, H.J., and Tsai, H.J. (2009) Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs. Nucleic acids research. 37(10):e77
Tu, C.T., Yang, T.C., and Tsai, H.J. (2009) Nkx2.7 and Nkx2.5 function redundantly and are required for cardiac morphogenesis of zebrafish embryos. PLoS One. 4(1):e4249
Huang, H.Y., Dai, E.S., Liu, J.T., Tu, C.T., Yang, T.C., and Tsai, H.J. (2009) The embryonic expression patterns and the knockdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(1):232-240
Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D. (2008) The orphan G protein-coupled receptor 161 is required for left-right patterning. Developmental Biology. 323(1):31-40
Grimes, A.C., Erwin, K.N., Stadt, H.A., Hunter, G.L., Gefroh, H.A., Tsai, H.J., and Kirby, M.L. (2008) PCB126 Exposure Disrupts Zebrafish Ventricular and Branchial but Not Early Neural Crest Development. Toxicological sciences : an official journal of the Society of Toxicology. 106(1):193-205
Lee, H.C., Tsai, J.N., Liao, P.Y., Tsai, W.Y., Lin, K.Y., Chuang, C.C., Sun, C.K., Chang, W.C., and Tsai, H.J. (2007) Glycogen synthase kinase 3alpha and 3beta have distinct functions during cardiogenesis of zebrafish embryo. BMC Developmental Biology. 7(1):93
Holtzman, N.G., Schoenebeck, J.J., Tsai, H.J., and Yelon, D. (2007) Endocardium is necessary for cardiomyocyte movement during heart tube assembly. Development (Cambridge, England). 134(13):2379-2386
Auman, H.J., Coleman, H., Riley, H.E., Olale, F., Tsai, H.J., and Yelon, D. (2007) Functional Modulation of Cardiac Form through Regionally Confined Cell Shape Changes. PLoS Biology. 5(3):e53
Ho, Y.L., Lin, Y.H., Tsai, I.J., Hsieh, F.J., and Tsai, H.J. (2007) In Vivo Assessment of Cardiac Morphology and Function in Heart-specific Green Fluorescent Zebrafish. Journal of the Formosan Medical Association = Taiwan yi zhi. 106(3):181-186
Chen, Y.H., Wang, Y.H., Chang, M.Y., Lin, C.Y., Weng, C.W., Westerfield, M., and Tsai, H.J. (2007) Multiple upstream modules regulate zebrafish myf5 expression. BMC Developmental Biology. 7(1):1
Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J. (2006) Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology. 299(2):594-608
Hu, C.Y., Yang, C.H., Chen, W.Y., Huang, C.J., Huang, H.Y., Chen, M.S., and Tsai, H.J. (2006) Egr1 gene knockdown affects embryonic ocular development in zebrafish. Molecular Vision. 12:1250-1258
Sabaawy, H.E., Azuma, M., Embree, L.J., Tsai, H.J., Starost, M.F., and Hickstein, D.D. (2006) TEL-AML1 transgenic zebrafish model of precursor B cell acute lymphoblastic leukemia. Proceedings of the National Academy of Sciences of the United States of America. 103(41):15166-15171
Sato, M., Tsai, H.J., and Yost, H.J. (2006) Semaphorin3D regulates invasion of cardiac neural crest cells into the primary heart field. Developmental Biology. 298(1):12-21
Chen, S.Y., Hsieh, C.S., Chu, S.W., Lin, C.Y., Ko, C.Y., Chen, Y.C., Tsai, H.J.,Hu, C.H., and Sun, C.K. (2006) Noninvasive harmonics optical microscopy for long-term observation of embryonic nervous system development in vivo. Journal of Biomedical Optics. 11(5):54022
Forouhar, A.S., Liebling, M., Hickerson, A., Nasiraei-Moghaddam, A., Tsai, H.J., Hove, J.R., Fraser, S.E., Dickinson, M.E., and Gharib, M. (2006) The embryonic vertebrate heart tube is a dynamic suction pump. Science (New York, N.Y.). 312(5774):751-753
Wang, Y.H., Chen, Y.H., Lu, J.H., Lin, Y.J., Chang, M.Y., and Tsai, H.J. (2006) Epidermis-restricted expression of zebrafish cytokeratin II is controlled by a -141/+85 minimal promoter, and cassette -141/-111 is essential for driving the tissue specificity. Differentiation; research in biological diversity. 74(4):186-194
Tsai, T.H., Lin, C.Y., Tsai, H.J., Chen, S.Y., Tai, S.P., Lin, K.H., and Sun, C.K. (2006) Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section. Optics letters. 31(7):930-932
Wang, Y.H., Chen, Y.H., Lin, Y.J., and Tsai, H.J. (2006) Spatiotemporal expression of zebrafish keratin 18 during early embryogenesis and the establishment of a keratin 18:RFP transgenic line. Gene expression patterns : GEP. 6(4):335-339
Lee, H.C., Huang, H.Y., Lin, C.Y., Chen, Y.H., and Tsai, H.J. (2006) Foxd3 mediates zebrafish myf5 expression during early somitogenesis. Developmental Biology. 290(2):359-372
Wang, Y.H., Chen, Y.H., Lu, J.H., and Tsai, H.J. (2005) A 23-amino Acid motif spanning the basic domain targets zebrafish myogenic regulatory factor myf5 into nucleolus. DNA and cell biology. 24(10):651-660
Huang, C.J., Jou, T.S., Ho, Y.L., Lee, W.H., Jeng, Y.T., Hsieh, F.J., and Tsai, H.J. (2005) Conditional expression of a myocardium-specific transgene in zebrafish transgenic lines. Developmental Dynamics : an official publication of the American Association of Anatomists. 233(4):1294-1303
Sun, C.K., Chu, S.W., Chen, S.Y., Tsai, T.H., Liu, T.M., Lin, C.Y., and Tsai, H.J. (2004) Higher harmonic generation microscopy for developmental biology. Journal of structural biology. 147(1):19-30
Lin, C.Y., Chen, Y.H., Lee, H.C., and Tsai, H.J. (2004) Novel cis-element in intron 1 represses somite expression of zebrafish myf-5. Gene. 334:63-72
Shu, X., Cheng, K., Patel, N., Chen, F., Joseph, E., Tsai, H.-J., and Chen, J.-N. (2003) Na,K-ATPase is essential for embryonic heart development in the zebrafish. Development (Cambridge, England). 130:6165-6173
Hsiao, C.D., Ekker, M., and Tsai, H.J. (2003) Skin-specific expression of ictacalcin, a homolog of the S100 genes, during zebrafish embryogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 228(4):745-750
Hsiao, C.-D. and Tsai, H.-J. (2003) Transgenic zebrafish with fluorescent germ cell: a useful tool to visualize germ cell proliferation and juvenile hermaphroditism in vivo. Developmental Biology. 262:313-323
Raya, A., Koth, C.M., Büscher, D., Kawakami, Y., Itoh, T., Raya, R.M., Sternick, G., Tsai, H.J., Rodriguez-Esteban, C., and Izpisúa Belmonte, J.C. (2003) Activation of Notch signaling pathway precedes heart regeneration in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. (Suppl. 1) 100:11889-11895
Huang, C.-J., Tu, C.-T., Hsiao, C.-D., Hsieh, F.-J., and Tsai, H.-J. (2003) Germ-line transmission of a myocardium-specific GFP transgene reveals critical regulatory elements in the cardiac myosin light chain 2 promoter of zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 228(1):30-40
Chen, Y.-H., Lee, H.-C., Liu, C.-F., Lin, C.-Y., and Tsai, H.-J. (2003) Novel regulatory sequence -82/-62 functions as a key element to drive the somite-specificity of zebrafish myf-5. Developmental Dynamics : an official publication of the American Association of Anatomists. 228(1):41-50
Hsiao, C.-D., Tsai, W.-Y., Horng, L.-S., and Tsai, H.-J. (2003) Molecular structure and developmental expression of three muscle-type troponin T genes in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 227:266-279
Hsiao, C.D., Tsai, W.Y., and Tsai, H.J. (2002) Isolation and expression of two zebrafish homologues of parvalbumin genes related to chicken CPV3 and mammalian oncomodulin. Mechanisms of Development. (Suppl.) 119:S161-S166
Chen, Y.-H. and Tsai, H.-J. (2002) Treatment with Myf5-morpholino results in somite patterning and brain formation defects in zebrafish. Differentiation; research in biological diversity. 70(8):447-456
Ho, Y.-L., Shau, Y.-W., Tsai, H.-J., Lin, L.-C., Huang, P.-J., and Hsieh, F.-J. (2002) Assessment of zebrafish cardiac performance using Doppler echocardiography and power angiography. Ultrasound Med. Biol.. 28(9):1137-1143
Wang, T.-M., Chen, Y.-H., Liu, C.-F., and Tsai, H.-J. (2002) Functional analysis of the proximal promoter regions of fish rhodopsin and myf-5 genes using transgenesis. Marine biotechnology (New York, N.Y.). 4(3):247-255
Chen, Y.-H., Liang, C.-T., and Tsai, H.-J. (2002) Expression, purification and DNA-binding activity of tilapia muscle-specific transcription factor, MyoD, produced in Escherichia coli. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 131(4):795-805
Hsiao, C.D., Hsieh, F.J., and Tsai, H.J. (2001) Enhanced expression and stable transmission of transgenes flanked by inverted terminal repeats from adeno-associated virus in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 220(4):323-336
Chen, Y.-H., Lee, W.-C., Liu, C.-F., and Tsai, H.-J. (2001) Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene. Genesis (New York, N.Y. : 2000). 29(1):22-35
Chen, Y.-H., Lee, W.-C., Cheng, C.-H., and Tsai, H.-J. (2000) Muscle regulatory factor gene: zebrafish (Danio rerio) myogenin cDNA. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 127(1):97-103

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