ZFIN Image: Lee et al., 2022, Figure 2

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Figure 2

Western blot analysis of ANP32a in zebrafish embryos undergoing either knockdown or overexpression of Anp32a. (A) Diagrams were used to depict base-pairings among Anp32a mRNA, antisense morpholino oligonucleotides (MO) of Anp32a (Anp32a-MO) and wobble nucleotides of Anp32a-flag mRNA (wobble-Anp32a-flag mRNA). (B) Western blot analysis. Zebrafish embryos at one-cell stage were microinjected with Anp32a-MO (MO), wobble-Anp32a-flag mRNA (wb-RNA) and MO plus wobble mRNA (MO + wb-RNA) as indicated, followed by extraction of total embryonic proteins at 48 hpf. Protein levels of endogenous ANP32a and exogenous ANP32a-flag were detected using antibodies against ANP32a and reporter protein flag. ?-tubulin served as an internal control. Protein levels relative to each internal control are presented below each lane.

Acknowledgments

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