ZFIN Image: Lee et al., 2022, Figure 7

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Figure 7

Overexpression of Anp32a in SCI embryos of zebrafish could induce the active proliferation of radial glial cells around the SCI site. Fluorescence-activated cell sorting (FACS) and a cell cycle analysis of radial glial cells in Anp32a-overexpressing SCI embryos of zebrafish were performed. Anp32a mRNA was microinjected into one-cell stage embryos from Tg(gfap:GFP) zebrafish, followed by SCI at 48 hpf and DAPI staining at 24 hpi. (A) Scatter profiles of Anp32a-overexpressing Tg(gfap:GFP) SCI-embryonic cells. All analytic cells were gated at P1; GFP-positive cells were gated at P2; DAPI-labeled-positive cells were gated at P6; GFP-negative cells were gated at P4. (B) Typical FACS dataset. The number shown in the right corner is the percentage of proliferated cells at each gate relative to the total examined cells in each group. (C) Cell cycle analysis from DAPI-labeled-positive cells shown on P6.

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