Morpholino

MO1-nanog

ID

ZDB-MRPHLNO-120403-3

Name

MO1-nanog

Previous Names

MO1-zgc:193933
nanog-like-MO1 (1)

Target

nanog (1)

Sequence

5' - CTGGCATCTTCCAGTCCGCCATTTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Translation-blocking MO.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO1-nanog

Expressed Gene	Anatomy	Figures
camsap2a	(not) yolk syncytial layer	Fig. 3 , Fig. 7 from Xu et al., 2012
chrd	whole organism	Fig 2 from He et al., 2020
ddx4	primordial germ cell whole organism	Fig. 6, Fig. 7 from Liu et al., 2017 Fig. 6 from Wang et al., 2016
dharma	whole organism	Fig 2 from He et al., 2020
dkk1b	whole organism	Fig 3 from He et al., 2020
emx1	presumptive telencephalon whole organism	Fig 2 , Fig 3 from He et al., 2020
foxi1	ectoderm ventral region	Fig 2 from He et al., 2020
frzb	whole organism	Fig 3 from He et al., 2020
gata5	margin dorsal region	Fig. 2 , Fig. 7 from Xu et al., 2012
gata6	margin (not) yolk syncytial layer	Fig. 3 , Fig. 7 from Xu et al., 2012
hnf4a	(not) yolk syncytial layer	Fig. 3 , Fig. 7 from Xu et al., 2012
mixl1	margin dorsal region	Fig. 2 , Fig. 7 from Xu et al., 2012
mxtx2	(not) yolk syncytial layer	Fig. 3 from Xu et al., 2012
nanog	whole organism	Fig 2 from He et al., 2020
ndr1	margin dorsal region	Fig. 2 , Fig. 7 from Xu et al., 2012
ndr2	margin dorsal region	Fig. 2 , Fig. 7 from Xu et al., 2012
nlk1	whole organism	Fig. 5 from Liu et al., 2017
otx2b	ectoderm dorsal region	Fig 2 from He et al., 2020
six3b	forebrain neural keel whole organism	Fig 2 , Fig 3 from He et al., 2020
slc26a1	(not) yolk syncytial layer	Fig. 3 , Fig. 7 from Xu et al., 2012
sox17	margin dorsal region	Fig. 2 from Xu et al., 2012
sox32	margin dorsal region	Fig. 2 , Fig. 7 from Xu et al., 2012
sp5l	whole organism	Fig 3 from He et al., 2020

Phenotype

Phenotype resulting from MO1-nanog

Phenotype	Fish	Figures
blastomere displaced to blastoderm dorsal region, abnormal	TU + MO1-nanog	Fig. 1 from Xu et al., 2012
canonical Wnt signaling pathway increased efficacy, abnormal	WT + MO1-nanog	Fig 3 from He et al., 2020
chromatin organization process quality, abnormal	TU + MO1-nanog	Fig. 4 from Liu et al., 2018
ectoderm dorsal region otx2b expression increased distribution, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
ectoderm ventral region foxi1 expression decreased distribution, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
forebrain morphology, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
forebrain neural keel six3b expression decreased amount, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
gastrulation disrupted, abnormal	TU + MO1-nanog	Fig. 1 from Xu et al., 2012
margin constricted, abnormal	TU + MO1-nanog	Fig. 1 from Xu et al., 2012
negative regulation of canonical Wnt signaling pathway decreased occurrence, abnormal	WT + MO1-nanog	Fig 3 from He et al., 2020
presumptive telencephalon emx1 expression decreased amount, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
primordial germ cell increased amount, abnormal	AB + MO1-nanog	Fig. 6 from Wang et al., 2016
primordial germ cell mislocalised, abnormal	AB + MO1-nanog	Fig. 6 from Wang et al., 2016
telencephalon morphology, abnormal	WT + MO1-nanog	Fig 3 from He et al., 2020
whole organism six3b expression decreased amount, abnormal	WT + MO1-nanog	Fig 3 from He et al., 2020
whole organism emx1 expression decreased amount, abnormal	WT + MO1-nanog	Fig 3 from He et al., 2020
whole organism nanog expression decreased amount, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
whole organism nlk1 expression decreased amount, abnormal	WT + MO1-nanog	Fig. 5 from Liu et al., 2017
whole organism frzb expression decreased amount, abnormal	WT + MO1-nanog	Fig 3 from He et al., 2020
whole organism dkk1b expression decreased amount, abnormal	WT + MO1-nanog	Fig 3 from He et al., 2020
whole organism dorsalized, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
whole organism chrd expression increased amount, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
whole organism dharma expression increased amount, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
whole organism ddx4 expression increased amount, abnormal	AB + MO1-nanog	Fig. 6 from Liu et al., 2017 Fig. 6 from Wang et al., 2016
whole organism sp5l expression increased distribution, abnormal	WT + MO1-nanog	Fig 3 from He et al., 2020
whole organism dharma expression increased distribution, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
whole organism chrd expression increased distribution, abnormal	WT + MO1-nanog	Fig 2 from He et al., 2020
whole organism lacks parts or has fewer parts of type presumptive endoderm, abnormal	TU + MO1-nanog	Fig. 2 from Xu et al., 2012
whole organism cell ddx4 expression increased distribution, abnormal	WT + MO1-nanog	Fig. 7 from Liu et al., 2017
yolk broken, abnormal	TU + MO1-nanog	Fig. 1 from Xu et al., 2012
yolk syncytial layer morphology, abnormal	TU + MO1-nanog	Fig. 3 from Xu et al., 2012
yolk syncytial layer nucleus decreased amount, abnormal	TU + MO1-nanog	Fig. 3 from Xu et al., 2012

Phenotype of all Fish created by or utilizing MO1-nanog

Phenotype	Fish	Conditions	Figures
primordial germ cell increased amount, abnormal	AB + MO1-nanog	standard conditions	Fig. 6 from Wang et al., 2016
whole organism ddx4 expression increased amount, abnormal	AB + MO1-nanog	standard conditions	Fig. 6 from Wang et al., 2016
primordial germ cell mislocalised, abnormal	AB + MO1-nanog	standard conditions	Fig. 6 from Wang et al., 2016
gastrulation disrupted, abnormal	TU + MO1-nanog	standard conditions	Fig. 1 from Xu et al., 2012
yolk syncytial layer morphology, abnormal	TU + MO1-nanog	standard conditions	Fig. 3 from Xu et al., 2012
chromatin organization process quality, abnormal	TU + MO1-nanog	standard conditions	Fig. 4 from Liu et al., 2018
margin constricted, abnormal	TU + MO1-nanog	standard conditions	Fig. 1 from Xu et al., 2012
whole organism lacks parts or has fewer parts of type presumptive endoderm, abnormal	TU + MO1-nanog	standard conditions	Fig. 2 from Xu et al., 2012
yolk syncytial layer nucleus decreased amount, abnormal	TU + MO1-nanog	standard conditions	Fig. 3 from Xu et al., 2012
yolk broken, abnormal	TU + MO1-nanog	standard conditions	Fig. 1 from Xu et al., 2012
blastomere displaced to blastoderm dorsal region, abnormal	TU + MO1-nanog	standard conditions	Fig. 1 from Xu et al., 2012
whole organism six3b expression decreased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 3 from He et al., 2020
ectoderm ventral region foxi1 expression decreased distribution, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
forebrain morphology, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
forebrain neural keel six3b expression decreased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
whole organism dorsalized, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
whole organism chrd expression increased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
whole organism dharma expression increased distribution, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
presumptive telencephalon emx1 expression decreased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
whole organism chrd expression increased distribution, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
whole organism nlk1 expression decreased amount, abnormal	WT + MO1-nanog	standard conditions	Fig. 5 from Liu et al., 2017
whole organism cell ddx4 expression increased distribution, abnormal	WT + MO1-nanog	standard conditions	Fig. 7 from Liu et al., 2017
whole organism dharma expression increased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
canonical Wnt signaling pathway increased efficacy, abnormal	WT + MO1-nanog	standard conditions	Fig 3 from He et al., 2020
whole organism emx1 expression decreased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 3 from He et al., 2020
negative regulation of canonical Wnt signaling pathway decreased occurrence, abnormal	WT + MO1-nanog	standard conditions	Fig 3 from He et al., 2020
whole organism dkk1b expression decreased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 3 from He et al., 2020
whole organism nanog expression decreased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
ectoderm dorsal region otx2b expression increased distribution, abnormal	WT + MO1-nanog	standard conditions	Fig 2 from He et al., 2020
whole organism ddx4 expression increased amount, abnormal	WT + MO1-nanog	standard conditions	Fig. 6 from Liu et al., 2017
telencephalon morphology, abnormal	WT + MO1-nanog	standard conditions	Fig 3 from He et al., 2020
whole organism frzb expression decreased amount, abnormal	WT + MO1-nanog	standard conditions	Fig 3 from He et al., 2020
whole organism sp5l expression increased distribution, abnormal	WT + MO1-nanog	standard conditions	Fig 3 from He et al., 2020
head absent, abnormal	WT + MO1-nanog + MO1-tcf7l1a	standard conditions	Fig 3 from He et al., 2020
whole organism frzb expression amount, ameliorated	WT + MO1-nanog + MO1-wnt8a + MO2-wnt8a	standard conditions	Fig 3 from He et al., 2020
whole organism emx1 expression amount, ameliorated	WT + MO1-nanog + MO1-wnt8a + MO2-wnt8a	standard conditions	Fig 3 from He et al., 2020
whole organism dkk1b expression amount, ameliorated	WT + MO1-nanog + MO1-wnt8a + MO2-wnt8a	standard conditions	Fig 3 from He et al., 2020
whole organism six3b expression increased amount, abnormal	WT + MO1-nanog + MO1-wnt8a + MO2-wnt8a	standard conditions	Fig 3 from He et al., 2020
whole organism sp5l expression position, ameliorated	WT + MO1-nanog + MO1-wnt8a + MO2-wnt8a	standard conditions	Fig 3 from He et al., 2020

Citations