ZFIN ID: ZDB-FIG-160512-11
Perner et al., 2016 - Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning. Journal of visualized experiments : JoVE   (110):e53921 Full text @ J. Vis. Exp.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 4

Knockdown of wt1a Disrupts Embryonic Kidney Development. Representative, extended depth of focus images from time-lapse recordings. In control morpholino injected embryos, kidney development shows normal progress with growing tubules and nephron primordia which start to fuse at the midline. In contrast, wt1a morphants fail to form proper nephron primordia and a massive amount of GFP positive cells are outside of the pronephric field. (np, nephron primordium; pt, pronephric tubule).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP li1Tg control 20-25 somites pronephric glomerulus ISH
20-25 somites pronephric tubule ISH
Prim-5 pronephric glomerulus ISH
Prim-5 pronephric tubule ISH
li1Tg + MO2-wt1a standard conditions 20-25 somites somite ISH
Prim-5 somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
li1Tg + MO2-wt1a standard conditions 20-25 somites pronephros development disrupted, abnormal
20-25 somites somite EGFP expression mislocalised, abnormal
20-25 somites somite EGFP expression spatial pattern, abnormal
Prim-5 pronephros development disrupted, abnormal
Prim-5 somite EGFP expression mislocalised, abnormal
Prim-5 somite EGFP expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of visualized experiments : JoVE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Vis. Exp.