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Fig. 6

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Song et al., 2026 - Formononetin attenuates glucocorticoid-induced osteoporosis via multiple osteoimmunological pathways: evidence from zebrafish and network pharmacology
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Fig. 6

FMN rescues the osteoporosis-related core targets and osteogenic functional markers in the Pred-induced zebrafish osteoporosis model. Gene expression levels of osteogenic factor sp7 (A) and network pharmacology-related factors calm3, egfr, gsk3β, jun, and ptgs2 (B-F), as determined by RT-qPCR; osteogenic markers (OCN and ALP) as determined by ELISA analysis (G-H). Bar graphs represent the mean ± standard error of the mean (SEM) from three independent experiments. Significant differences compared to the control group are marked as: # (p < 0.05), ## (p < 0.01), #### (p < 0.0001); compared to the model group: * (p < 0.05), ** (p < 0.01), *** (p < 0.001), **** (p < 0.0001); ns indicates no statistically significant difference

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