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Lubash et al., 2026 - RNA Polymerase III subunit Polr3a is required for craniofacial cartilage and bone development in zebrafish

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Fig 7 Single cell RNA-sequencing reveals transcriptional upregulation of genes in polr3a^sa907/sa907 zebrafish at 3 dpf.
(A) UMAP plot of aggregated mutant and wild-type cells. (B) Volcano plot of differentially expressed genes from a pseudo-bulk analysis of polr3a^sa907/sa907 cells (mutant) compared to wild-type control cells (wt). (C) Volcano plot of differentially expressed genes in polr3a^sa907/sa907 versus controls in the cartilage cluster. (D) qRT-PCR of genes in the integrated stress response pathway show about a 1.5-fold increase in mutants at 3 dpf. atf4b ****p < 0.0001, chac1 **p = 0.006, aars ***p = 0.0003. (E) qRT-PCR for genes in the Tp53 pathway (mdm2, tp53, and cdkn1a) show a nearly 3-fold increase in mutants at 3 dpf. ****p < 0.0001.

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