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Figure Caption

Figure 5

Effect of T3 and IOP treatments upon opsin mRNA expression in 5 dpf zebrafish larvae. (A–F) No significant statistical differences were observed when comparing the control (Ctrl) to T3-treated and IOP-treated groups. Each bar represents the mean fold change ± SEM from four independent experiments, and each sample was analyzed in duplicate. Ribosomal 18S and actin mRNA were used as reference genes to apply the 2−ΔΔCT formula. Statistical analysis was performed using one-way ANOVA with multiple comparisons and Tukey post hoc tests.

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Acknowledgments
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