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Fig. 3 VD3 regulates the folate transport and metabolism in zebrafish. The gene expression of slc19a1 (A), slc46a1 (B), and folr1 (C) in the intestine, kidney, and liver of the zebrafish fed with 0 or 800 IU/kg VD3 diets for 3 wk was analyzed. The gene expression of dhfr and mthfr related to folate one-carbon metabolism in the intestine (D) and liver (E) of the zebrafish fed with 0 or 800 IU/kg VD3 diets for 3 wk was analyzed. F: the gene expression of fpgs and ggh in the liver was analyzed. G: the folate content of liver was measured with microbiological assay. Data are presented as means ± SE (n ≥ 6 fish). *P < 0.05; **P < 0.01; ns, no statistical significance. VD, vitamin D.