PUBLICATION
Vitamin D promotes the folate transport and metabolism in zebrafish (Danio rerio)
- Authors
- Liang, S., Zhang, H., Jiao, L., Shao, R., Lan, Y., Liao, X., Mai, K., Ai, Q., Wan, M.
- ID
- ZDB-PUB-240208-1
- Date
- 2024
- Source
- American journal of physiology. Endocrinology and metabolism 326(4): E482-E492 (Journal)
- Registered Authors
- Keywords
- Cyp2r1, Folate, Folic acid, Gut microbiota, Vitamin D3
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents
- Folic Acid/metabolism
- Folic Acid/pharmacology
- Reduced Folate Carrier Protein/genetics
- Reduced Folate Carrier Protein/metabolism
- Vitamin D*
- Vitamin D Deficiency*
- Vitamins
- Zebrafish
- PubMed
- 38324257 Full text @ Am. J. Physiol. Endocrinol. Metab.
Citation
Liang, S., Zhang, H., Jiao, L., Shao, R., Lan, Y., Liao, X., Mai, K., Ai, Q., Wan, M. (2024) Vitamin D promotes the folate transport and metabolism in zebrafish (Danio rerio). American journal of physiology. Endocrinology and metabolism. 326(4):E482-E492.
Abstract
Vitamin D(VD) is a fat-soluble sterol that possesses a wide range of physiological functions. The present study aimed to evaluate the effects of VD on folate metabolism in zebrafish and further investigate the underlying mechanism. Wild-type (WT) zebrafish were fed with the diet containing 0 IU/kg VD3 or 800 IU/kg VD3 for 3 weeks. Meanwhile, cyp2r1 mutant zebrafish with impaired VD metabolism served as another model of VD deficiency. Our results showed that VD deficiency in zebrafish suppressed the gene expression of folate transporters, including reduced folate carrier (RFC) and proton-coupled folate transporter (PCFT) in the intestine. Moreover, VD influenced the gene expression of several enzymes related to cellular folate metabolism in the intestine and liver of zebrafish. Importantly, VD-deficient zebrafish contained a remarkably lower level of folate content in the liver. Notably, VD was incapable to alter folate metabolism in zebrafish when gut microbiota was depleted by antibiotic treatment. Further studies proved that gut commensals from VD-deficient fish displayed a lower capacity to produce folate than those from WT fish. Our study revealed the potential correlation between VD and folate metabolism in zebrafish, and gut microbiota played a key role in VD-regulated folate metabolism in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping