|
Fig. 2 The expression of the genes related to the folate pathway in different tissues and developmental stages of zebrafish. The gene expression of folate transporters and metabolic enzymes in zebrafish at early developmental stages (A) and in different tissues of adult zebrafish (3 mpf) (B). The gene expression of slc19a1 (C), folr1 (D), slc46a1 (E), dhfr (F), and mthfr (G) in the intestine of wild-type zebrafish was assayed at 1, 2, 3, 4, and 6 mpf. The expression levels of the target genes are relative to the levels at 1 mpf. H: the folate content in the visceral mass of wild-type zebrafish at 2, 3, 4, and 6 mpf. Data are presented as means ± SE (n ≥ 5 fish). Different letters indicate significant differences (P < 0.05). dhfr, dihydrofolate reductase; folr1, folate receptor α; fpgs, folylpolyglutamate synthase; ggh, γ-glutamyl hydrolase; mpf, months post fertilization; mthfr, methylenetetrahydrofolate reductase; slc19a1, reduced folate carrier; slc46a1, proton-coupled folate transporter.