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Liao et al., 2023 - Disruption of sirtuin 7 in zebrafish facilitates hypoxia tolerance

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Figure 7

Zebrafish sirt7 facilitates ubiquitination of hif-1αa, hif-1αb, hif-2αa, and hif-2αb.A–D, quantitative real-time PCR (qPCR) analysis of the mRNA levels of hif-1αa (A), hif-1αb (B), hif-2αa (C), or hif-2αb (D) in sirt7-null mutant (sirt7^{ihblqs4/ihblqs4}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). E–H, qPCR analysis of the mRNA levels of hif-1αa (E), hif-1αb (F), hif-2αa (G), or hif-2αb (H) in sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). I–L, ubiquitination analysis of hif-1αa (I), hif-1αb (J), hif-2αa (K), or hif-2αb (L) in HEK293T cells transfected with indicated plasmids for 24 h and then treated with MG132 (20 μM) for 8 h. p Values were calculated by two-way ANOVA analysis (A–H); ns, not significant; data based on one representative experiment performed in three biological replicates from at least three independent experiments (mean ± SD).

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.