|
Figure 3
Exposure to CPF modulates THs levels and metabolism in zebrafish ovaries. (a) Ovarian fT3 levels (O-fT3) were measured by ELISA assay in adult ovaries from Vehicle and exposed females (n = 5 ovaries/group), as described in M&M section. (b–g) Levels of the mRNAs of T3 responsive genes (igfbp1a and esr1) and enzymes involved in THs metabolism (dio1, dio2, dio3a, and dio3b) in ovaries of zebrafish exposed to CPF. RT-qPCR tests were performed on five biological samples (n = 5 ovaries/group). Data are reported as fold change values calculated as a ratio between average relative gene expression in exposed and control ovaries after normalization on tubaI mRNA. Significant differences are indicated with * p < 0.05 and ** p < 0.01 using Student’s t-test.