ZFIN Image: He et al., 2021, Fig. 3

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Figure Caption

Fig. 3 Fig. 3. Gene expression profiling of foxc1a nju18 hearts. A: A flow chart of the heart collection and RNA extraction process. B: GO analysis shows significant GO terms. C: KEGG pathway analysis shows enriched KEGG terms obtained. D: A heatmap of 28 selected differentially expressed genes from the RNA sequencing data. The color blocks indicate the direction and degree of the fold change (log2[FC] values). E: Whole-mount in situ hybridization (WISH) and quantitative real-time PCR (qRT-PCR) of 72 hpf and 96 hpf hearts show the expression patterns of wwtr1. Sample size of WISH: WT (n = 5), foxc1a nju18 (n = 3) at 72 hpf; WT (n = 7), foxc1a nju18 (n = 8) at 96 hpf. All images are ventral views with the head oriented upward. F: The qRT-PCR evaluating the expression levels of Notch signaling related genes in the hearts of foxc1a nju18 mutants and WT siblings. ∗, P < 0.05; ∗∗, P < 0.01. Error bars represent the standard deviation.

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Reprinted from Journal of genetics and genomics = Yi chuan xue bao, 49(6), He, L., Zhang, Q., Jiang, D., Zhang, Y., Wei, Y., Yang, Y., Li, N., Wang, S., Yue, Y., Zhao, Q., Zebrafish foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression, 559-568, Copyright (2021) with permission from Elsevier. Full text @ J. Genet. Genomics