ZFIN Image: Shi et al., 2022, Figure 2

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Figure Caption

Figure 2

cyp17a2-deficiency caused pituitary pomca upregulation and interrenal gland enlargement in zebrafish larvae. WISH analyses with probes of pomca(A1, A2), cyp11a1(B1, B2), cyp21a2(C1, C2) and hsd3b1(D1, D2) in cyp17a2+/+ and cyp17a2-/- fish at 5 dpf. WISH analyses with probes of pomca(E1–E4), cyp11a1(F1–F4), cyp21a2(G1–G4), and hsd3b1(H1–H4) in fish at 5 dpf. (E1, F1, G1, H1)cyp17a2+/+;pomca+/+ fish. (E2, F2, G2, H2)cyp17a2-/-;pomca+/+ fish. (E3, F3, G3, H3)cyp17a2+/+;pomca-/- fish. (E4, F4, G4, H4)cyp17a2-/-;pomca-/- fish. Black, red, and green arrows indicate normal, enlarged, and decreased interrenal gland, respectively. (I) Bar chart represents the quantification and statistical analyses of WISH signals in pituitary and interrenal gland of fish at 5 dpf. (J1, J2, K1, K2) WISH analysis using the probe of cyp21a2 in cyp17a2+/+ and cyp17a2-/- fish administrated with DMSO or HC from 1 dpf to 5 dpf. (J1, J2) DMSO. (K1, K2) HC. (L) Bar chart represents the quantification and statistical analyses of WISH signals in interrenal gland of fish administrated with DMSO or HC. HC, hydrocortisone. DMSO, dimethyl sulfoxide. n.s., no significant difference. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.

Acknowledgments

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