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Fig. 3
Effect of rig-I mRNA overexpression on primitive erythropoiesis and myelopoiesis
(A?C) Expression of pu.1/spi1 at the six-somite stage in control MO (A)?, rig-I MO (B)?, or rig-I MO and mRNA (C)?injected embryos from posterodorsal view with anterior to the top. (D?F) Expression of lcp1/l-plastin at 24 hpf in control MO (D)?, rig-I MO (E)?, or rig-I MO and mRNA (F)?injected embryos from lateral view. (G?I) Expression of mpx at 36 hpf in control MO (G)?, rig-I MO (H)?, or rig-I MO and mRNA (I)?injected embryos from lateral view. (J?L) Expression of gata-1 at the six-somite stage in control MO (J)?, rig-I MO (K)?, or rig-I MO and mRNA (L)?injected embryos from posterodorsal view with posterior to the top. Images were captured under Olympus stereoscope (MVX10 MacroView; original magnification ×50). (M?P) Percent of embryos with phenotype of normal or decreased staining intensity in control or rig-I morphant embryos from (A)?(L) based on WISH analysis. The number of embryos used for statistics is shown in each figure. Each experiment was repeated three times (n = 3, mean ± SD, Student t test, **p < 0.01, ***p < 0.001; ns, not significant).