Fig. S4
Fig. S4
mpp10 is essential for the organogenesis of digestive organs.
(A) Whole-mount in situ hybridization (WISH) using the mpp10 probe on WT unfertilized eggs and embryos at 6hpf, 12hpf, and 1dpf to 5dpf. (B) Upper panel: diagram showing the mpp10 genomic DNA. Red bar, exons; blue line, introns. Lower panels: showing the DNA sequence changes in mpp10Δ10 and mpp10+2 two mutant alleles which are mutated in the exon2 of the mpp10 gene (red letters, deletion; blue letters, insertion). (C) Representative images showing the overall morphology of a 5dpf-old WT and mpp10Δ10 (mΔ10). Black arrow, cardiac region; red arrow, swimming bladder; blue arrow, eye. (D-G) WISH using the prox1 (D) and hhex (E) probes on 30hpf- and 50hpf-old embryos, and the gata6 (F) and foxa3 (G) probes on 30hpf-, 50hpf- and 3dpf-old embryos. The number of total embryos genotyped (as denominator) and the number of embryos exhibiting the displayed phenotype (as numerator) are shown at the bottom