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Fig. 5

Exposure to estradiol (18 nM) by immersion caused a significant increase in mRNA expression of ligands (A) or receptor (B) genes, in the brain of juvenile zebrafish. sGnRHa, zfNKBa, zfNKBb, zfNKF, or senktide (see legend to Fig. 4 for details), were injected intraperitoneally to mature zebrafish and blood was collected 6 h thereafter (C). Hormone values are means ± SEM. Statistical significance vs. corresponding control values: **P < 0.01; *P < 0.05.

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