ZFIN Image: Khaliq et al., 2015, Fig. 2

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Figure Caption

Fig. 2

The expression patterns of id1, id2b, id3, and id4 during liver development in zebrafish. (A–D) Wild-type embryos were processed for WISH analysis with id1 (A), id2b (B), id3 (C), and id4 (D) probes. id1 appears to be ubiquitously expressed at 30 hpf, but its expression is absent in the liver at 48 and 72 hpf. id2b and id3 expression in the liver was detected at 72 hpf (arrows), but not at 30 or 48 hpf. id4 is not expressed in the liver. Dorsal views, anterior to the left. (E) id1 in situ hybridization (red), combined with anti-GFP immunostaining (green), in Tg(sox17:GFP) embryos reveals id1 expression in the liver-forming region (brackets), but not in the dorsal pancreas (dotted lines) at 30 hpf. Single confocal optical section, ventral view, anterior up. Scale bars: 100 (A–D), 20 (E) µm.

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Expression / Labeling details

Acknowledgments

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Reprinted from Mechanisms of Development, 138 Pt 3, Khaliq, M., Choi, T.Y., So, J., Shin, D., Id2a is required for hepatic outgrowth during liver development in zebrafish, 399-414, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.