Fig. 2
Fig. 2
UXT is essential for zebrafish angiogenesis. (A-C) RT-PCR verification of angiogenesis markers in 18hpf, 24hpf or 30hpf zebrafish embryos injected with 4ng of control morpholino (Ctrl MO) or with 4ng of UXT morpholino (UXT MO). Data shown are the meanĀ±s.e.m. (at least three independent experiments); *P<0.05, **P<0.01 versus the corresponding control. (D) Whole-mount in situ hybridization of embryos injected with 4ng of control morpholino (Ctrl MO) or 4ng of UXT morpholino (UXT MO). Riboprobes against the angiogenesis marker kdrl, the pronephric marker pax2.1 and the erythroid marker gata1 were visualized at 22hpf. The red arrowhead indicates the decreased expression of kdrl in UXT-deficient embryos.