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Fig. 6 Regulation of lfng expression. (A-F) Dorsal views of control MO (A,B) and triple ascl1a, ascl1b and ngn1 MO (E,F) injected embryos, or DAPT (C,D) treated embryos at 16 hpf showing lfng (A,C,E) and deltaA (B,D,F) expression in the hindbrain. Arrowheads in A-D indicate the expression of lfng and deltaA in the middle of each rhombomere. (G-N) Dorsal views of control (G-J) and ngn1 RNA (K-N) injected embryos at ∼3-somite stage showing lfng (G,I,K,M) and deltaA (H,J,L,N) expression in the anterior and posterior neural plate. Scale bars: 100 μm in F for A-F; 200 μm in N for G-N.
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