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Fig. 1 Expression pattern of lfng in the zebrafish hindbrain. (A-E) Dorsal views of wild-type embryos following in situ hybridisation to detect lfng transcripts in the hindbrain between 14 and 40 hpf. Ovals indicate the otocyst, arrowheads in C indicate the location of hindbrain boundaries and double arrows in D and E indicate the neurogenic zones that form adjacent to boundaries. (F,G) Confocal images within the plane of the ventricular zone showing dorsal views of a 40 hpf embryo following detection of lfng (red) and deltaA (green) mRNA combined with anti-N-Cadherin (blue) and DAPI (grey) staining to reveal the cell surface and nucleus, respectively. Arrowheads indicate cells in which lfng and deltaA are coexpressed. VZ, ventricular zone. (H,I) Transverse sections of 40 hpf embryos following fluorescent in situ hybridisation to detect expression of lfng (red) in comparison to deltaA (H, green) and deltaB (I, green) combined with DAPI staining (grey). Dotted line indicates the boundary between the ventricular and mantle zones. Scale bars: 100 μm in A for A-E; 10 μm in F for F,G; 50 μm in H for H,I.