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Fig. 3

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ZDB-IMAGE-090127-3
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Figures for Batista et al., 2008
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Fig. 3 Zebrafish spinal cord contains both V2a and V2b cells. Lateral views of wild-type trunks at 24 h. Double in situ hybridisation showing gata3 (A, D and E), gata2 (B and C), lhx3 (F and H), and chx10 (G) expression in red and chx10 (C and D), gata3 (B and H), vsx1 (E and F), scl (A) and lhx3 (G) in green. Single channel views and merged images are shown. Anterior is left, dorsal is up. Scale bar = 50 μm. panel I shows a single focal plane magnified image of the dashed white box in panel A. Panel J shows a single focal plane magnified image of the dashed white box in panel B. Panel K shows a single focal plane magnified image of the dashed white box in panel G. Merged images are on the left, followed by the single green channel and then the single red channel. Cell outlines are traced in white. In all cases, stars in merged images indicate double-labelled cells.

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Reprinted from Developmental Biology, 322(2), Batista, M.F., Jacobstein, J., and Lewis, K.E., Zebrafish V2 cells develop into excitatory CiD and Notch signalling dependent inhibitory VeLD interneurons, 263-275, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.