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Fig. 2 Adult germ line deficient gonads exhibit expression of genes normally expressed in wild-type testes. (A) RT-PCR of genes normally expressed in the ovary and/or testis. Each experiment was done on two independent samples. Negative controls lacking reverse transcriptase are in alternating lanes (c). (B-C) Detection of 3βHSD activity in Leydig cells using a chromatographic assay. (B) Wild-type and (C) germ line deficient testes have 3βHSD activity in intertubular regions where Leydig cells reside. Scale bars are 10 μm.
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Reprinted from Developmental Biology, 324(2), Siegfried, K.R., and Nüsslein-Volhard, C., Germ line control of female sex determination in zebrafish, 277-287, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.