Gene Trap Construct

Tg(GAL4-VP16,UAS:EGFP)

ID

ZDB-GTCONSTRCT-070314-1

Previous Names

SAGVG (1)
Gt(Gal4-VP16,UAS:EGFP)
Gt(Gal4-VP16;UAS:EGFP)
Tg(Gal4-VP16,UAS:EGFP)

Type

engineered_plasmid

Regulatory Regions

UAS (1)

Coding Sequences

EGFP (1)
GAL4 (1)
VP16 (1)

[Em λ][Ex λ]

Fpbase:EGFP

Contains Sequences

None

Note

A Tol2 transposon-based, bipartite construct consisting of a Gal4-VP16 gene/enhancer trap as well as a cis-linked UAS-regulated eGFP reporter. The vector contains a splice acceptor sequence derived from the rabbit beta-globin gene (275 base pairs of intron 2 and 45 base pairs of exon 3) which was amplified from the pT2KXIG”IN vector (Urasaki et al., 2006) and cloned upstream of tandem Gal4-VP16 and UAS:eGFP elements (Koster and Fraser, 2001). The entire unit was subcloned into the pT2KXIG”IN vector to be flanked by a 535 bp right Tol2 arm and a 517 bp left Tol2 arm.

Plasmid Map

None

Genomic Features

That Utilize Tg(GAL4-VP16,UAS:EGFP) Construct

Genomic Feature	Affected Genomic Regions
as31Gt	icam3
as38Tg
c215Tg
c218Tg
c220Gt	ikbkg
c223Tg
c228Tg
c229Tg
c230Tg
c233Tg
c235Tg
c236Tg
c237Tg
c239Tg
c240Tg
c241Tg
c247Tg
c251Tg
c269Tg
ub120Tg
ub121Tg
ub148Tg
ub14Tg
ub15Tg
ub17Tg
ub28Tg
ub38Tg
ub39Tg
ub3Tg
ub43Tg
ub44Tg
ub45Tg
ub46Tg
ub49Tg
ub61Tg
ub65Tg
ub67Tg
ub68Tg
ub79Tg
ub80Tg
ub95Tg

Transgenics

That Utilize Tg(GAL4-VP16,UAS:EGFP) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
as38Tg			Fig. 3 from Liu et al., 2020
c215Tg (AB)
c218Tg (AB)			Fig. 1 from Goll et al., 2009
c223Tg (AB)			Fig. 1 from Goll et al., 2009
c223Tg/+; c264Tg/+		Fig. 5 from Davison et al., 2007
c228Tg (AB)
c229Tg (AB)
c230Tg (AB)
c230Tg/+; c264Tg/+		Fig. 5 from Davison et al., 2007
c233Tg (AB)
c235Tg (AB)
c236Tg (AB)
c237Tg (AB)
c239Tg (AB)
c240Tg (AB)			Fig. 1 from Goll et al., 2009
c241Tg (AB)
c247Tg (AB)
c251Tg (AB)			Fig. 1 from Goll et al., 2009
c264Tg; ub3Tg		Fig. 7 from Zhao et al., 2009	Fig. 7 from Zhao et al., 2009
c264Tg; ub43Tg		Fig. 7 from Zhao et al., 2009	Fig. 7 from Zhao et al., 2009
c269Tg			Fig. 5 from Feng et al., 2010
c269Tg (AB)			2 figures from Goll et al., 2009
icam3^as31Gt	icam3	Fig. 2 from Hsieh et al., 2018	Fig. 1 from Hsieh et al., 2018
icam3^as31Gt; cy13Tg	icam3		Fig. 1 from Hsieh et al., 2018
icam3^as31Gt; y1Tg	icam3
ikbkg^{c220Gt/c220Gt} (AB)	ikbkg	Fig. S2 from Davison et al., 2007	Fig. 1 from Goll et al., 2009
ikbkg^c220Gt (AB)	ikbkg
s1993tTg; ub43Tg
ub120Tg
ub120Tg/+ (AB)
ub120Tg; zf153Tg			Fig. 1 from Zhao et al., 2009
ub121Tg			3 figures from Gupta et al., 2011
ub121Tg/+ (AB)
ub148Tg			2 figures from Gupta et al., 2011
ub148Tg/+ (AB)
ub148Tg; zf153Tg			Fig. 4 from Gupta et al., 2011
ub14Tg			2 figures from Gupta et al., 2011
ub14Tg/+ (AB)
ub14Tg; zf153Tg			Fig. 4 from Gupta et al., 2011
ub15Tg			2 figures from Gupta et al., 2011
ub15Tg/+ (AB)
ub17Tg			2 figures from Gupta et al., 2011
ub17Tg/+ (AB)
ub17Tg; zf153Tg			Fig. 4 from Gupta et al., 2011
ub28Tg			2 figures from Gupta et al., 2011
ub28Tg/+ (AB)
ub38Tg			3 figures from Gupta et al., 2011
ub38Tg/+ (AB)
ub39Tg			4 figures from Gupta et al., 2011
ub39Tg/+ (AB)
ub3Tg			5 figures from Zhao et al., 2009
ub3Tg/+ (AB)
ub3Tg; zf153Tg
ub43Tg			5 figures from Zhao et al., 2009
ub43Tg/+ (AB)
ub43Tg; zf153Tg
ub44Tg			2 figures from Gupta et al., 2011
ub44Tg/+ (AB)
ub45Tg			3 figures from Gupta et al., 2011
ub45Tg/+ (AB)
ub46Tg
ub49Tg			2 figures from Gharbi et al., 2012
ub49Tg; zf153Tg
ub61Tg			3 figures from Gupta et al., 2011
ub61Tg/+ (AB)
ub65Tg			4 figures from Gupta et al., 2011
ub65Tg/+ (AB)
ub65Tg; zf153Tg			Fig. 4 from Gupta et al., 2011
ub67Tg			2 figures from Gupta et al., 2011
ub67Tg/+ (AB)
ub68Tg
ub79Tg			4 figures from Gupta et al., 2011
ub79Tg/+ (AB)
ub80Tg			2 figures from Gupta et al., 2011
ub80Tg/+ (AB)
ub80Tg; zf153Tg			Fig. 4 from Gupta et al., 2011
ub95Tg			2 figures from Gupta et al., 2011
ub95Tg/+ (AB)
ub95Tg; zf153Tg			Fig. 4 from Gupta et al., 2011
uhrf1^{hi272Tg/hi272Tg}; c269Tg	uhrf1		Fig. 5 from Feng et al., 2010

Sequence Information

Other Construct Pages

None

Citations