PUBLICATION

Distinct expression of two foxg1 paralogues in zebrafish

Authors
Zhao, X.F., Suh, C.S., Prat, C.R., Ellingsen, S., and Fjose, A.
ID
ZDB-PUB-090424-15
Date
2009
Source
Gene expression patterns : GEP   9(5): 266-272 (Journal)
Registered Authors
Ellingsen, Ståle, Fjose, Anders
Keywords
Forkhead, Fox, Foxg1, Zebrafish, Transgenic, Enhancer trap, Gal4, UAS, Development, Paralogues, Eye, Retina, Ear, Optic nerve, CMZ, Confocal
MeSH Terms
  • Protein Isoforms/genetics
  • Forkhead Transcription Factors/genetics*
  • Gene Expression Profiling*
  • Animals, Genetically Modified
  • Molecular Sequence Data
  • Prosencephalon/embryology
  • Prosencephalon/metabolism
  • Zebrafish Proteins/genetics*
  • Ear/embryology
  • Gene Expression Regulation, Developmental*
  • Microscopy, Fluorescence
  • Animals
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Sequence Homology, Amino Acid
  • Eye/embryology
  • Eye/metabolism
  • Retina/embryology
  • Retina/metabolism
  • Amino Acid Sequence
  • In Situ Hybridization
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
(all 23)
PubMed
19379839 Full text @ Gene Expr. Patterns
Abstract
The forkhead proteins (Fox) act as transcription factors in many biological processes in a wide range of species. One member of this superfamily, Foxg1, has essential roles in the development of eyes, telencephalon, ears and olfactory system. Zebrafish foxg1 has been reported to have similar roles as the mouse orthologue Foxg1. However, no data has been reported about possible zebrafish foxg1 paralogues. In this study we identified one zebrafish foxg1 paralogue by enhancer trapping, which we designate foxg1b. A more diverged paralogue, foxg1c, was identified by homology searches. Sequence comparisons indicate that both foxg1b and foxg1c are less related to mouse than the previously characterized foxg1. We report that foxg1b is expressed in a regionally restricted pattern within the developing eye, mainly in the dorsal-nasal retina, which is similar to the retinal expression of mouse Foxg1. By contrast, foxg1cis only expressed transiently in the eyes and forebrain between 14 and 20 hours post-fertilization, while expression was detectedexclusively in the developing inner ear at later stages. Our results suggest that foxg1b and foxg1c have undergone expression pattern divergence during evolution that has resulted in functional specialization.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ub120TgTransgenic Insertion
    zf153TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      ub120Tg
      ub120Tg; zf153Tg
      WT
      zf153Tg
      1 - 4 of 4
      Show
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      RFPEFGRFP
      1 - 2 of 2
      Show
      Mapping
      Entity Type Entity Symbol Location
      Featureub120TgUnmapped
      GENEfoxg1bChr: 11 Details
      1 - 2 of 2
      Show
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      Citation
      Zhao, X.F., Suh, C.S., Prat, C.R., Ellingsen, S., and Fjose, A. (2009) Distinct expression of two foxg1 paralogues in zebrafish. Gene expression patterns : GEP. 9(5):266-272.