Fig. 1

Characterization of the fzd9b 22 bp deletion (fzd9b^22bpDEL) line. A) BLAST alignment of the predicted amino acids (aa) sequences for wildtype Fzd9b (WT, top) and the products predicted from the mutant allele (bottom), showing the region of exon 1 used for genotyping (see Sup. Fig. 2). Red circle marks the mutation starting site, altered residues are in red and stop codon is shown as a dash (-). B) Relative gene expression (log₁₀ scale) of fzd9b in 5 dpf larvae; each point represents a pool of 16 larvae (N = 4 per genotype); bars show mean ± standard error mean (SEM). C) Quantification of the whole mount in situ hybridization (ISH) for cmyb at the tail of 40 hpf embryos (N = 7 per genotype); right panel shows representative images. D) Quantification of neuronal branch points following acetylated tubulin immunostaining and skeleton analysis in larval tails; graphs show individual values and mean ± SEM; representative images are shown alongside with yellow rectangles depicting areas analysed. E) qPCR analysis of wnt5a, wnt5b, wnt9a, scn4aa, scn4ab and tafa5l expression in the same 5 dpf larval pools used in panel B; graphs show box-and-whiskers (min to max) with mean as a central line; dots represent biological replicates. Statistical details are provided in Sup. Table 1. In all cases: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000 vs. corresponding pairwise comparison; Scale bars represent 25 µm