FIGURE

Fig. 3

ID

ZDB-FIG-260716-56

Publication

Wang et al., 2026 - Checkpoint kinase 2 coordinates autophagy activation and Aurora kinase A degradation to regulate primary cilia for cell invasion

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Fig. 3

Zebrafish chek2 is a maternal transcript required for primary ciliation during embryonic development. A Zebrafish chek2 transcript appears as a maternal transcript that later changed to zygotic expression at the anterior region determined by whole-mount in situ hybridization and (B) RT-PCR (upper row, chek2; lower row, actin). The hpf means hours post fertilization. All embryos are in lateral view, with the animal pole to the top (0.75 hpf), dorsal (4–10 hpf) to the right, and anterior (10 and 24 hpf) to the left. C The sgRNA and the morpholino (MO) binding sites in the chek2 cDNA locus. D Primary cilia stained with acetylated tubulin (Ac-tub) antibody in the head primordium (2500 µm2) at the bud stage. E Cells labeled with DAPI that contain primary cilia were calculated as the percentage. Embryo numbers in each injection from the left to the right were (GFP+Cas9 mRNA: 19, chek2 sgRNA+Cas9 mRNA:13, Control MO:21, chek2 MO: 13, chek2 MO+ human chek2 mRNA: 12, human chek2 mRNA: 9)

Expression Data

Gene:	chek2
Antibody:	Ab1-tuba
Fish:	AB AB + CRISPR3-chek2 AB + MO1-chek2
Knockdown Reagents:	CRISPR3-chek2 MO1-chek2
Anatomical Terms:	head head cilium whole organism
Stage Range:	2-cell to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR3-chek2 AB + MO1-chek2
Knockdown Reagents:	CRISPR3-chek2 MO1-chek2
Observed In:	head cilium head cilium organization
Stage:	Bud

Phenotype Detail

Acknowledgments

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