FIGURE

FIGURE 3

ID

ZDB-FIG-260626-14

Publication

Rezi et al., 2026 - ZDHHC5 interacts physically and functionally with DLG1 at primary cilia and regulates ciliary length and kidney morphology

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FIGURE 3

Ciliary localization of ZDHHC5. (A) IFM analysis of ciliary localization of ZDHHC5 in control (WT) and Dlg1 knockout cells without (KO) or with (Rescue) stable re-expression of mCherry-DLG1. Cells were cultured for 24 h in supplement-free medium and subjected to IFM analysis using antibodies against ZDHHC5 (green) and acetylated α-tubulin (AcTub; magenta) to stain the ciliary axoneme. Insets show zoom-ins of the cilium-basal body axis. Note that average ciliary length is unaffected by DLG1 loss under these conditions (Rezi et al., 2024). (B) Quantification of mean fluorescence intensity (MFI) of ZDHHC5 in cilia of the indicated cell lines, based on images as in A. (C) Western blot analysis of whole cell lysate from the indicated cell lines using antibodies listed to the right of the blots. Molecular mass markers are shown in kDa to the left. GAPDH serves as loading control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol