FIGURE 3

FIGURE 3

Ciliary localization of ZDHHC5. (A) IFM analysis of ciliary localization of ZDHHC5 in control (WT) and Dlg1 knockout cells without (KO) or with (Rescue) stable re-expression of mCherry-DLG1. Cells were cultured for 24 h in supplement-free medium and subjected to IFM analysis using antibodies against ZDHHC5 (green) and acetylated α-tubulin (AcTub; magenta) to stain the ciliary axoneme. Insets show zoom-ins of the cilium-basal body axis. Note that average ciliary length is unaffected by DLG1 loss under these conditions (Rezi et al., 2024). (B) Quantification of mean fluorescence intensity (MFI) of ZDHHC5 in cilia of the indicated cell lines, based on images as in A. (C) Western blot analysis of whole cell lysate from the indicated cell lines using antibodies listed to the right of the blots. Molecular mass markers are shown in kDa to the left. GAPDH serves as loading control.