Fig. 6

Global activity in response to the dark-light preference test in 8 dpf larvae prenatally exposed. A. Average movement of control larvae across the dark-light preference test protocol. A reduction in movement is observed during the strobe-light section. B. Smoothing of the average response for each condition across the entire experiment and a linear mixed-model analysis was performed for each phase separately. There was a reduction in log2 fold change over time in all groups, except in the EM one that did not reach significance. C. Average log2 fold change relative to controls during the strobe light section. All conditions displayed a reduction in movement in the light zone, especially in the EM-exposed larvae. D. Average log2 fold change relative to controls during the last 10 min of light following the strobe light section. E, M, EN and EMN-exposed animals showed significantly reduced activity compared to controls. Although not significant, animals in the N and MN groups also tended to display lower activity levels. B: * p < 0.05 compared to control animals. P-values were obtained using a linear mixed-effects model with condition over time as a fixed effect and subject (larva ID) and Batch as random effects. C-D: * = p < 0.05 compared to controls. Evaluated using mixed-effects models, including Batch as a random effect, followed by Benjamini–Hochberg correction for multiple comparisons. n ≥ 15 per condition.