Fig. 8

Thermal response to heat in 8 dpf larvae prenatally exposed. A. Average locomotor responses over time of control larvae throughout the thermosensation assay. B. Smoothing of the average response for each condition across the entire experiment and a linear mixed-model analysis was performed. Larvae in the E, M and EM groups showed an enhanced average fold-change over time, while N-exposed groups showed a reduction in responsiveness to heat. C. Average Log2 fold change relative to controls during the whole experiment. M-exposed larvae were hyperactive compared to controls, while the opposite response was observed in the N-exposed animals. B: * p < 0.05 compared to control animals. P-values were obtained using a linear mixed-effects model with condition over time as a fixed effect and subject (larva ID) and Batch as random effects. C: * = p < 0.05 compared to controls. Evaluated using mixed-effects models, including Batch as a random effect, followed by Benjamini–Hochberg correction for multiple comparison. n ≥ 8 per condition.