Fig. 9

Thermal response to heat in 14 dpf larvae prenatally exposed. A. Average locomotor responses over time of control larvae throughout the thermosensation assay. B. Average Log2 fold change relative to controls for the duration of the entire experiment. EMN-exposed larvae were hyperactive compared to controls, while the opposite was measured in the M-exposed animals. C. Nearly all the EMN-exposed larvae were hyperactive, while most of the E- and M-exposed ones were hypoactive during the whole experiment. B: * = p < 0.05 compared to controls. Evaluated using mixed-effects models, including Batch as a random effect, followed by Benjamini–Hochberg correction for multiple comparisons. C: * = p < 0.05 compared to control animals. P-values were calculated using Firth's bias-reduced logistic regression to compare each treatment to control, followed by Benjamini–Hochberg correction for multiple comparisons. n ≥ 6 per condition.