FIGURE

Fig 6

ID

ZDB-FIG-260514-19

Publication

McCarthy et al., 2026 - The impact of cannabidiol (CBD) in hyperglycemic zebrafish (Danio rerio)

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Fig 6

Western Blot analysis of inflammatory pathway and tight junction proteins.

No statistically significant differences across treatment + exposure groups were observed for (A) phosphorylated JNK_46, phosphorylated AKT, or (B) phosphorylated JNK₅₄ in retinal homogenates. However, tissue from the Glucose + CBD group did show an average 4x increase in pJNK/JNK₅₄ (B) and a 2x increase in pAKT-AKT (A). Analysis of protein levels of the tight junction proteins claudin-5 and occludin (C) revealed opposite trends, with Glucose and Glucose + CBD fish showing an average upregulation of occludin and an average downregulation in claudin-5. Biological replicates for AKT, pAKT, pAKT/AKT n = 6. Biological replicates for Occludin, JNK₅₄, JNK_46, pJNK₅₄, pJNK_46, pJNK/JNK₅₄, pJNK/JNK₄₆ n = 4. Biological replicates for claudin-5 n = 3. Individual data points from biological replicates are shown. Condition = treatment + exposure groups.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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