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ERG peak amplitude and time to amplitude for a- b- and d-wave. (A) Mean ERG trace showing the photoreceptor a-wave, ON-bipolar cell b-wave, and OFF-bipolar cell d-wave components. (B) a-wave peak amplitude. Pairwise comparison showed significantly larger a-waves in the Glucose + CBD group compared to Control (p = 0.003). (C) Time to peak a-wave ERG response. Pairwise analysis showed a significantly delayed implicit time for the a-wave response between Glucose + CBD and all other treatment groups (Control p < 0.001, Vehicle p < 0.001, Drug Control p = 0.015, Mannitol + CBD p = 0.044, Hyperglycemia p = 0.020). (D) ERG b-wave peak amplitude response. Both Control and Drug Control groups had significantly greater b-wave amplitudes than all other treatment groups (Vehicle, Mannitol + CBD, Hyperglycemia, Glucose + CBD; p ≤ 0.001 for all). Glucose + CBD had a significantly lower b-wave amplitude than Mannitol + CBD (p = 0.046). (E) Time to peak b-wave ERG response. Responses from Control fish had significantly delayed b-wave response times compared to all other treatment groups (Drug Control, Vehicle, Mannitol + CBD, Hyperglycemia, Glucose + CBD; p ≤ 0.001 all). (F) ERG d-wave amplitude. The Control group had a significantly higher d-wave amplitude compared to all other treatment groups except Drug Control (Vehicle, Hyperglycemia, Glucose + CBD; p < 0.001 for all, Mannitol + CBD; p = 0.023). Hyperglycemic fish also had smaller peak amplitudes than the Drug Control (p = 0.013) (G) Time to peak d-wave ERG response. Glucose + CBD had significantly slower times to peak than all other treatment groups (p < 0. 001). Control n = 30, Vehicle n = 24, Drug Control n = 30, Mannitol + CBD n = 24, Hyperglycemia n = 35, Glucose + CBD n = 26. In (B) through (G) box and whisker plots show the distribution of biological replicates. * < 0.05, ** < 0.01, and *** < 0.001.
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