Fig 1

Overview of applied methods.

Zebrafish were exposed in one of six treatment + exposure groups (left) over 4-weeks. For the first 2 weeks, a 1% Glucose (or Mannitol) treatment solution was used; for the next 2 weeks a 2% solution was used. CBD exposure occurred every other day when the animal was coming out of the treament solution (Glucose, Mannitol, or Water). After 4 weeks, behavioral recordings of optomotor responses (OMR) were obtained, followed by electroretinograms (ERGs) which analyzed retinal function. Retinal and liver tissue were then collected for qPCR and Western Blot analysis. CBD and Methanol (vehicle for CBD) were administered at a final concentration of 5 mg/ml and 0.005%, respectively.