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Morphology and numbers of short-wavelength-sensitive photoreceptor subtypes in the absence of miR-183 at 10 months old WTTL and miR-183−/− fish. (A) Immunostaining with anti-dre-UV-opsin antibody of retinal flat mounts (left) and cross sections (right). Nuclei and UV cones are depicted in light grey (DAPI) and light blue respectively. The KO retina shows fewer UV cones and decreased length and staining of the cone outer segments. (B) Fluorescent image of retinal cross sections with anti-dre-blue-opsin. The KO retina shows fewer blue cones and decreased length and staining of cone outer segments. (C) Statistical results of UV and blue cone numbers. The value indicates the number of cones per 100 μm in an 8 μm thick section. (D) Cone outer segment lengths for UV and Blue cones. (E) Normalized fluorescence intensity for UV and Blue opsins. WT: N = 5. miR-183−/−: N = 6. Bars in the graph represent s.e.m. * p < 0.05, ** p < 0.01, *** p < 0.001. Student’s t-test.
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