Metronidazole sensitivity, oxygen consumption rates, and mechanoelectrical transduction function following MnTBAP treatment. (A) Percent lethality of zebrafish embryos after 24-h exposure to 10 mM metronidazole with and without cotreatment with 50 μM MnTBAP. (B) Representative images of 5-days post fertilization zebrafish ercc6−/− larvae (24 h after metronidazole exposure) treated with MnTBAP or untreated. Data is from 3 replicate experiments, with 15 fish per group, per experiment. Each data point represents percent survival of 15 fish. (C) Basal oxygen consumption rates of 5-days post fertilization larvae with and without MnTBAP treatment. Data is from 4 replicate experiments with 60 embryos per group, per experiment. Each data point represents a value averaged from 60 pooled larvae. (D) Relative fluorescence intensity of FM1–43 uptake in MnTBAP treated 5-days post fertilization wildtype and ercc6−/− larvae, with and without 30 s UVC exposure. (E) Representative fluorescence images showing FM1–43 uptake for untreated wildtype and ercc6−/− larvae, wildtype and ercc6−/− larvae after UVC treatment, and wildtype and ercc6−/− larvae with UVC and MnTBAP treatment. Data is from 3 replicate experiments with a total of 5–10 larvae per group, and each data point represents a single zebrafish larva. All data is represented by mean ± SD. Groups were compared via two-way ANOVA with multiple comparisons (*P-value<0.05; **P-value<0.01; ***P-value<0.001; ****P-value<0.0001). Scale bar: 500 μm.
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