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The role of gper1 in chronic skin inflammation is tissue-specific, as its overexpression in basal keratinocytes improves skin condition. a Schematic of the experimental procedure used. Zebrafish lines of interest were crossed to obtain eggs overexpressing gper1 in either neutrophils or basal keratinocytes. A control group without overexpression was also obtained. At 2 dpf, proliferation was analyzed by BrdU assay. At 3 dpf, images were taken to analyze KC aggregates and neutrophils distribution. b Representative images of 3 dpf control larvae and larvae with gper1-overexpressing neutrophils. c Quantification of KC aggregates number in skin in both groups. d Quantification of neutrophil distribution in the tail in every condition. e Representative images of 3 dpf control larvae and larvae with gper1-overexpressing basal KC. f Number of KC aggregates in skin in every group. g Representative images of BrdU assay in 2 dpf control larvae and larvae with gper1-overexpressing basal KC. h Number of BrdU+ cells per area in skin in every group. Each dot represents one individual, and the means and SEM for each group are also shown. P values were calculated by Student’s t test. ns not significant; *P ≤ 0.05.
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