Figure 5

Strip1 modulates Hippo-pathway-related components and MST4 activity in NRVCMs. (a) Western blot analysis of Hippo pathway components in NRVCMs 72 h after infection with AdSTRIP1 (50 ifu) compared to AdLacZ control. (b–f) Densitometry of bands, normalized to β-actin and, for phosphorylated proteins, additionally to the respective total protein levels. Infection with 50 ifu AdSTRIP1 results in significantly increased (b) MOB1 phosphorylation, while total protein levels of (c–f) YAP, MST1, MST2 and TAZ are reduced significantly compared to control after 72 h. (g) Western blot analysis following STRIP1 knockdown (siSTRIP1) in NRVCMs shows a significant increase in phosphorylated MST4 (p-MST4), whereas total MST4 protein levels remain unchanged relative to control. Densitometric quantification of (h) p-MST4 relative to total MST4 and (i) relative to Ezrin, both normalized to β-actin. (a,g) Uncropped full-length blots are shown in Supplementary Figures S5 and S6; (a–i), n = 3; Student’s t-test; ns = not significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; Data are shown as mean ± SEM.