Figure 3

STRIP1 expression is reduced in human cardiomyopathy and models of mechanical stress. (a) Western blot analysis of Strip1 in human heart tissue from nonfailing (NF), DCM and ICM patients, with GAPDH as the loading control; (b) Densitometric quantification normalized to GAPDH shows significantly reduced STRIP1 expression in both DCM (n = 10 individuals) and ICM (n = 9) compared to nonfailing controls (n = 6); the representative blot does not show all biological replicates; (statistics: one-way ANOVA; * p < 0.05); (c) Western blot analysis of Strip1 in mouse heart samples reveals significantly decreased Strip1 protein levels following transverse aortic constriction (TAC) (n = 5) compared to sham-operated controls (n = 5), as quantified by (d) densitometry of the bands; (e) qPCR in NRVCMs after 48 h of mechanical stretch reveals significant downregulation of STRIP1 expression compared to unstretched control cells (n = 3); (a,c) Uncropped full-length blots are shown in Supplementary Figure S2; Student’s t-test; ns = not significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; Data are shown as mean ± SEM.