FIGURE

FIGURE 1

ID

ZDB-FIG-260327-8

Publication

Chen et al., 2026 - Sirt1 coordinates the mitochondrial UPR and myocellular proteostasis to preserve muscle integrity during muscle atrophy in zebrafish

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FIGURE 1

UPR^mt is activated during muscle atrophy in zebrafish larvae. (a) qRT-PCR analysis of dexamethasone (DEXA)-treated zebrafish larvae, showing the expression of the atrophy genes fbxo32 and trim63a (*p < 0.05, t-test, two-tailed, error bar indicates s.d.). (b) Bright-field images of myotome from DEXA-treated zebrafish larvae at 6 dpf. Arrow indicates disorganized muscle fibers. Scale bar represents 0.1 mm. (c) Fluorescence images of the UPR^mt reporter zebrafish treated with DEXA. Arrows indicate elevated fluorescent signals in the skeletal muscle. Scale bar represents 1 mm. (d) qRT-PCR analysis of DEXA-treated zebrafish larvae showing the expression of the UPR^mt genes hspd1, hspa9, lonp1, and clpp (*p < 0.05, t-test, two-tailed, error bar indicates s.d.). (e) Western blot and quantification of poly-ubiquitinated proteins in the detergent-soluble and -insoluble fractions of the protein lysates from DEXA-treated zebrafish larvae (*p < 0.05, t-test, two-tailed, error bar indicates s.d.).

Expression Data

Genes:	clpp fbxo32 hspa9 hspd1 lonp1 trim63a
Fish:	AB
Condition:	chemical treatment by environment: dexamethasone
Anatomical Term:	whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment by environment: dexamethasone
Observed In:	mitochondrial unfolded protein response muscle protein polyubiquitination whole organism
Stage:	Day 6

Phenotype Detail

Acknowledgments

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