FIGURE

Figure 4

ID

ZDB-FIG-260316-82

Publication

Wang et al., 2026 - Zebrafish PRL-3 Regulates Yolk Syncytial Layer Integrity and Actomyosin Contractility During Epiboly

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Figure 4

Whole-mount in situ hybridization reveals altered spatial patterns of no tail (ntl) and hatching gland 1 (hgg1) in zfPRL-3 morphants. Embryos injected with 4 ng control morpholino (MO) or zfPRL-3 MO were fixed at 8 h post fertilization and analyzed by whole-mount in situ hybridization. (A,B) Expression of ntl. (A) Control morphants exhibit a robust ntl marginal domain with a distinct animal pole–ward extension, whereas (B) zfPRL-3 morphants show a detectable ntl domain that appears more confined to the margin with reduced internal extension. (C,D) Expression of hgg1. (C) Control morphants display a compact hgg1-positive domain, while (D) zfPRL-3 morphants show a detectable but often weaker and less defined hgg1 signal. Scale bars = 250 μm.

Expression Data

Genes:	ctslb tbxta
Fish:	AB AB + MO1-ptp4a3a
Knockdown Reagent:	MO1-ptp4a3a
Anatomical Terms:	anterior axial hypoblast axial chorda mesoderm margin
Stage:	75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-ptp4a3a
Knockdown Reagent:	MO1-ptp4a3a
Observed In:	anterior axial hypoblast axial chorda mesoderm
Stage:	75%-epiboly

Phenotype Detail

Acknowledgments

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