ZFIN Image: Wang et al., 2026, Figure 4

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Figure Caption

Figure 4

Whole-mount in situ hybridization reveals altered spatial patterns of no tail (ntl) and hatching gland 1 (hgg1) in zfPRL-3 morphants. Embryos injected with 4 ng control morpholino (MO) or zfPRL-3 MO were fixed at 8 h post fertilization and analyzed by whole-mount in situ hybridization. (A,B) Expression of ntl. (A) Control morphants exhibit a robust ntl marginal domain with a distinct animal pole–ward extension, whereas (B) zfPRL-3 morphants show a detectable ntl domain that appears more confined to the margin with reduced internal extension. (C,D) Expression of hgg1. (C) Control morphants display a compact hgg1-positive domain, while (D) zfPRL-3 morphants show a detectable but often weaker and less defined hgg1 signal. Scale bars = 250 μm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.