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Changes of expression patterns of alx4a and grem1b in triple hox13 mutant larval zebrafish dorsal and anal primordia compared to wildtype. (A) Gene expression analysis of alx4a and grem1b using HCR v3.0 RNA-FISH in wildtype and triple hox13 mutant zebrafish at 5.4 and 5.6mm SL. alx4a expression is detected by the green fluorescence and grem1b expression is detected by the red fluorescence in the dorsal and anal fin primordia. Green and red arrowheads indicate the alx4a and grem1b domains of expression, respectively. White dotted lines indicate the anterior and posterior limits of each primordia. 5.4mm SL: n = 5 for both genotypes. 5.6mm SL: n = 4 for wildtype and n = 5 for triple hox13 mutants. Scale bars, 50 µm. (B) Average corrected total cell fluorescence (CTCF) for alx4a and grem1b in wildtype and triple hox13 mutants for each fin and SL listed in (A). CTCF graphs correspond to timepoints in adjacent panels in (A). Means + SE are presented for each group. Unpaired t-tests were performed to test for statistically significant changes, which are indicated as * (p < 0.05), ** (p < 0.01), or *** (p < 0.001).
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