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Triple hox13 mutants have proportionally smaller dorsal/anal fin primordia at 5.6mm SL, and smaller dorsal/anal fin base in adults. (A-D) Measurements of dorsal and anal fins at the base of the fin along the anterior-posterior axis of adult fish within 3-3.2 cm SL. Black dotted lines represent the distance measured. Scale bars, 1mm. (E-H) Measurement of length and width on wildtype dorsal and anal fin primordia at 5.6mm SL for wildtype and triple hox13 mutants at 5.6mm SL. The black vertical dotted lines represent the anterior and posterior limits of the fin. The red dotted lines represent the proximal and distal limits of the fin primordia. The length and width of the fins were measured between these respective limits. P = primordium, MFF = median fin fold. Scale bars, 200 µm. (I-J) Average length (anterior-posterior) at the base of the dorsal and anal fin in 3-3.2 cm SL wildtype and triple hox13 mutants. Measurements were averaged from n = 5 replicates for each group. Means + SE are presented. Unpaired t-tests were performed to test for statistically significant changes, which are indicated as * (p < 0.05), ** (p < 0.01), or *** (p < 0.001). (K-N) Average length and width of dorsal and anal fin primordia in 5.6mm SL wildtype and triple hox13 mutants. Measurements were averaged from n = 5 replicates for each group at each location. Means + SE are presented. Unpaired t-tests were performed to test for statistically significant changes, which are indicated as * (p < 0.05), ** (p < 0.01), or *** (p < 0.001).
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