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Triple homozygous hox13 mutants have thicker hemirays compared to wildtype siblings. (A) Reconstructed wildtype zebrafish dorsal fin from micro-CT scan. Black line indicates the location of the transverse section through the ray shown in (B). Scale bar, 700 µm. (B) Reconstructed cross section of wildtype dorsal fin hemirays. Red line indicates an example of measurements taken for (E). Scale bar, 150 µm. (C) Reconstructed cross section of triple hox13 mutant dorsal hemirays. Red lines indicated sample measurements taken for (E). Scale bar, 200 µm. (D) Traces of wildtype and triple hox13 mutant hemirays from reconstructed scans. Fin rays are named according to their position along the anterior-posterior axis (ex: A2 = second ray from the anterior side). Hemirays were observed at specific positions along the proximal distal axis shown on the left. These positions were selected for a consistent comparison between the wildtype and triple hox13 mutant rays and demonstrate that the changes are consistent along the A-P axis. SL = 3.2 cm for both fish. (E) Bar graphs of dorsal hemiray widths in µm of wild-type and triple hox13 mutants are presented at specific levels along the proximal distal axis. The ray of interest and distance from the base of the ray in µm is listed above each corresponding graph. Measurements were averaged from n = 3 replicates for each group at each location. Means + SE are presented. Unpaired t-tests were performed to test for statistically significant changes, which are indicated as * (p < 0.05), ** (p < 0.01), or *** (p < 0.001). A Mann-Whitney U test was performed for A4 fin ray (750 µm) since data was not normally distributed.
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